IGF1 Protein

Catalog No. ABIN7680475

This Recombinant IGF1 protein is produced in Escherichia coli (E. coli).

Quick Overview for IGF1 Protein (ABIN7680475)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Purity: More than 95 % as determined by SDS-PAGE. 

Grade: Animal-Free

Product Details

Purpose: rIGF-1 LR3 - Recombinant human Insulin-like growth factor-1 LR3

Characteristics: This product arrives as White lyophilized powder 

Endotoxin Level: Less than 5 EU/mg

Biological Activity Comment: The bioactivity determined by a cell proliferation assay using Balb/c 3T3 cell, corresponding to a specific activity of ≥ 1x10^6 Units/mg.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended to reconstitute LR3 IGF-1 at 100-200 μg/mL in sterile water.

Buffer: It is lyophilized with mannitol as the stabilizer.

Handling Advice: Use as soon as possible after opening. Avoid freeze-thaw cycles

Storage: -20 °C

Storage Comment: Stored at temperatures -20°C for at least 36 months. Further dilutions can be made in other aqueous buffer. Use as soon as possible after opening. Please avoid freeze-thaw cycles.

Expiry Date: 36 months

Target Details

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Alternative Name: Insulin-like growth factor-1

Background: Insulin-like growth factor-1 (IGF-1) is a hormone similar in molecular structure to insulin. It primarily functions through binding to its specific receptor, the insulin-like growth factor 1 receptor (IGF1R). IGF-1 is one of the most potent natural activators of the AKT signaling pathway, which stimulates cell growth and proliferation, and inhibits cell apoptosis. LR3 IGF-1 (Long Arg3 IGF-1) is an 83 amino acid analog of IGF-1 comprising the complete human IGF-1 sequence with the substitution of an Arg for the Glu at position 3 (hence R3), and a 13 amino acid extension peptide at the N-terminus. The LR3 IGF-1 has much higher stability and biological activity.

Molecular Weight: 9.1kD

Pathways: RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation